Analysis Note

ControlBrain tissue, Neuronal culture

Application

Research CategoryNeuroscience

Immunocytochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.

Immunohistochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.

Optimal working dilutions must be determined by the end user

Research Sub CategoryNeuronal & Glial MarkersNeurofilament & Neuron Metabolism

Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulinR06; is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.

Immunogen

Epitope: (Similar to TuJ1 antigen)

Synthetic peptides from human/rat Beta III Tubulin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1049

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
Four different antibodies were combined to make this product

ImmunoAffinity Purified

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot:
1:1000 dilution of this lot detected TUBULIN BETA III on 10 µg of mouse brain lysates.

Specificity

Recognizes Beta III Tubulin

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Target description

55 kDa